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KMID : 0363019880180020198
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Journal of Korean Academy of Periodontology
1988 Volume.18 No. 2 p.198 ~ p.210
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An Experimental Study on Arylsulfatase in Gingival Crevicular Fluid of Dogs with Periodontal Disease
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Lee In-Kyu
Kim Hyung-Seop
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Abstract
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It is diagnostically interesting to measure the enzyme activity as an indicator of the patient´s present state of periodontal disease. In addition, it is also useful to consider the relationship between enzyme activity and clinical index. The arylsulfatase activity was measured and compared with volume of gingival crevicular fluid and clinical index. The purpose of this study was to show the diagnostic value of this enzyme. Experimental periodontitis was induced by ligating the left third and forth premolars of maxilla and mandible with wire from 4 dogs of 13-15kg Contralateral teeth were brushed 3 times a week as the control site. In two months, the filter paper strip was inserted into the gingival sulcus of the same teeth and gingival crevicular fluid was collected. And then, Periotron units and arylsulfatase activity were measured; sulcus bleeding index, plaque index, pocket depth, etc were also mesured. 1. Average values of arylsulfatase activity (optical density) were 0.043± 0.015 and 0.021± 0.006 at the experimental and control areas, respectively. 2. The relationship of periodontal clinical indices and Periotron units was statistically significant when the experimental and control areas were compared (p(0.01). 3. Correlation between arylsulfatase activity and Periotron units was statistically significant (p<0.01). 4. As the degree of sulcus bleeding index and the pocket depth increased, Periotron units and enzyme activity also increased.
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KEYWORD
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